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G-1 promotes Wnt/Hedgehog-signaling in the human primary <t>DPCs.</t> (A) mRNA expression levels of Wnt signaling-related genes in human hair follicle dermal papilla cells <t>(hDPCs)</t> stimulated with G-1 with or without G-36 measured by qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (B) Activation of the Wnt signaling-related protein β-Catenin in hDPCs stimulated with G-1, with or without G-36, measured using Western blotting ( n = 4). Internal controls: β-actin expression. (C) mRNA expression levels of Hedgehog signaling-related genes in hDPCs stimulated with G-1, with or without G-36, measured using qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (D) Expression levels of Hedgehog signaling-related proteins in hDPCs stimulated with G-1, measured using Western blotting ( n = 8). Internal controls: β-actin expression. * p < 0.05, ** p < 0.01 (Tukey–Kramer's post hoc test). All data are presented as the means ± SE.
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G-1 promotes Wnt/Hedgehog-signaling in the human primary <t>DPCs.</t> (A) mRNA expression levels of Wnt signaling-related genes in human hair follicle dermal papilla cells <t>(hDPCs)</t> stimulated with G-1 with or without G-36 measured by qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (B) Activation of the Wnt signaling-related protein β-Catenin in hDPCs stimulated with G-1, with or without G-36, measured using Western blotting ( n = 4). Internal controls: β-actin expression. (C) mRNA expression levels of Hedgehog signaling-related genes in hDPCs stimulated with G-1, with or without G-36, measured using qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (D) Expression levels of Hedgehog signaling-related proteins in hDPCs stimulated with G-1, measured using Western blotting ( n = 8). Internal controls: β-actin expression. * p < 0.05, ** p < 0.01 (Tukey–Kramer's post hoc test). All data are presented as the means ± SE.
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hair follicle dermal papilla cell - by Bioz Stars, 2026-02
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G-1 promotes Wnt/Hedgehog-signaling in the human primary DPCs. (A) mRNA expression levels of Wnt signaling-related genes in human hair follicle dermal papilla cells (hDPCs) stimulated with G-1 with or without G-36 measured by qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (B) Activation of the Wnt signaling-related protein β-Catenin in hDPCs stimulated with G-1, with or without G-36, measured using Western blotting ( n = 4). Internal controls: β-actin expression. (C) mRNA expression levels of Hedgehog signaling-related genes in hDPCs stimulated with G-1, with or without G-36, measured using qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (D) Expression levels of Hedgehog signaling-related proteins in hDPCs stimulated with G-1, measured using Western blotting ( n = 8). Internal controls: β-actin expression. * p < 0.05, ** p < 0.01 (Tukey–Kramer's post hoc test). All data are presented as the means ± SE.

Journal: Frontiers in Pharmacology

Article Title: The GPR30 agonist G-1 promotes hair growth via Wnt/Hedgehog signaling in mice

doi: 10.3389/fphar.2025.1570922

Figure Lengend Snippet: G-1 promotes Wnt/Hedgehog-signaling in the human primary DPCs. (A) mRNA expression levels of Wnt signaling-related genes in human hair follicle dermal papilla cells (hDPCs) stimulated with G-1 with or without G-36 measured by qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (B) Activation of the Wnt signaling-related protein β-Catenin in hDPCs stimulated with G-1, with or without G-36, measured using Western blotting ( n = 4). Internal controls: β-actin expression. (C) mRNA expression levels of Hedgehog signaling-related genes in hDPCs stimulated with G-1, with or without G-36, measured using qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (D) Expression levels of Hedgehog signaling-related proteins in hDPCs stimulated with G-1, measured using Western blotting ( n = 8). Internal controls: β-actin expression. * p < 0.05, ** p < 0.01 (Tukey–Kramer's post hoc test). All data are presented as the means ± SE.

Article Snippet: Human hair follicle dermal papilla cells (hDPCs) were obtained from PromoCell (Heidelberg, Germany) and cultured in follicle dermal papilla cell growth medium (Promocell) supplemented with a growth medium Supplement Pack (Promocell).

Techniques: Expressing, Quantitative RT-PCR, Activation Assay, Western Blot